Part:BBa_K4785004:Design

pslg

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence of PslG was truncated to remove the signal peptide that was originally used to mediate the secretion of PslG in P. aeruginosa, so that PSLG could be better expressed in engineered bacteria.

Figure 1: The plasmids pET28a-6×His-PslG

Figure 2: The plasmids with the correct sequencing results were transferred into Escherichia coli BL21 for induced expression, then Escherichia coli was broken, and the target protein was purified by affinity chromatography and molecular sieve, and we obtained high purity of the target protein. (a), SDS-PAGE of PslG.

Source

This part is from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)v

References

Yu, S., Su, T., Wu, H. et al. PslG, a self-produced glycosyl hydrolase, triggers biofilm disassembly by disrupting exopolysaccharide matrix. Cell Res 25, 1352–1367 (2015). https://doi.org/10.1038/cr.2015.129